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The antibody is from purified rabbit serum by affinity purification via sequential chromatography on phospho-peptide and non-phospho-peptide affinity columns.
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Overexpression of this gene continues to be observed in many tumors, which results in chromosome instability, and therefore could contribute to tumorigenesis. This protein was found to affiliate with, and be involved in, the phosphorylation of NPAT protein (nuclear protein mapped into the ATM locus), which participates in mobile-cycle controlled histone gene expression and performs a important position in selling cell-cycle development inside the absence of pRB. Two alternatively spliced transcript variants of the gene, which encode distinctive isoforms, have been explained. Two added splice variants had been claimed but thorough nucleotide sequence information will not be but out there.
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Blocking peptides are peptides that bind precisely to the goal antibody and block antibody binding. These peptide ordinarily includes the epitope identified because of the antibody.
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This tutorial outlines even more achievable optimization of cellular and immunoassay parameters to be certain the absolute best final results are...
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This information outlines further achievable optimization of mobile and immunoassay parameters to make certain the very best effects are...
Antibodies sure to the blocking peptide now not bind into the epitope on the concentrate on protein. By evaluating the staining with the blocked antibody as opposed to the antibody by yourself, one can see which staining is certain.
Goal Info Cyclin E belongs towards the highly conserved cyclin spouse and children, whose users are characterized by a remarkable periodicity in protein abundance in the mobile cycle. Cyclins operate as regulators of CDK kinases. Distinct cyclins exhibit distinctive expression and degradation designs which add on the temporal coordination of each mitotic event. Cyclin E varieties a fancy with and functions for a regulatory subunit of CDK2, whose action is required for mobile cycle G1/S transition. This protein accumulates within the G1-S period boundary and it is degraded as cells development by means of S phase.
The measurement of protein phosphorylation is a useful gizmo for measuring the modulation of receptor activation by the two antibodies...
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